Hydrophobicity Of The Nadph Binding Domain Of Came

Biochimica et Biophysica Acta 1546 (2001) 71^78 www.bba-direct.com Hydrophobicity of the NADPH binding field of operation of camel genus Lens j-crystallin Mohammad D. Bazzi, Nayyar Rabbani, Ali S. Duhaiman * Department of Biochemistry, College of Science, ability Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia true 8 June 2000; reliable in revised form 10 October 2000; accepted 17 October 2000 Abstract Interaction of camel lens j-crystallin with the aquaphobic probe 1-anilinonaphthalene-8-sulfonic window glass (autonomic nervous system) enhanced the autonomic nervous system fluorescence and quenched the protein fluorescence. Both of these events were concentration-dependent and showed typical loudness curves suggesting specific ANS-j-crystallin binding. numerical analysis indicated that 1 mole j-crystallin ensnare at some 1 mole ANS. NADPH alone not 9,10-phenanthrenequinone (PQ) was able to remove j-crystallin-bound ANS. These results suggested the pre sence of a hydrophobic domain in j-crystallin, perchance at the NADPH binding commit. KCrystallin as wellhead as NADPH defend j-crystallin against thermal inactivation suggesting the importance of this site for enzyme stability. The NADPH:quinone oxidoreductase bodily function of j-crystallin was inhibited by ANS with NADPH as electron donor and PQ as electron acceptor.

Lineweaver-Burk plots indicated mixed-type ban with deference to NADPH, with a Ki of 2.3 WM. Secondary plots of inhibition with respect to NADPH indicated a dissociation ceaseless (KPI) of 12 WM for the j-crystallin-NADPH-ANS complex. The Ki b eing smaller than KPI suggested that compet! itive inhibition at the NADPH binding site was preponderant over non-competitive inhibition. the likes of ANS-j-crystallin binding, inhibition was dependent on ANS concentration but independent of incubation time. ß 2001 Elsevier Science B.V. completely rights reserved. Keywords: Camel lens; j-Crystallin; Hydrophobicity; Fluorescence; 1-Anilinonaphthalene-8-sulfonic blistery 1. Introduction j-Crystallin is an NADPH:quinone...If you want to get a all-inclusive essay, come in it on our website: BestEssayCheap.com

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